The Proteomics and Metabolomics Core Facility at the Redox Biology Center (RBC) supports faculty, scientists, clinicians, and students within the RBC, University of Nebraska-Lincoln, University of Nebraska Medical Center, and other universities and industries. The Facility is equipped with chromatography and mass spectrometry-based state-of-the-art technologies for proteomics and metabolomics (clinical and non-clinical) and offers personalized experimental design consultation and comprehensive, individualized bioinformatics support.
General services include:
- Small-molecule exact mass determination or quantitation using positive or negative ion modes, including MS/MS structural analysis;
- Protein identification using liquid chromatography-tandem mass spectrometry (LC/MS/MS) analysis and MASCOT and SEQUEST database search;
- Protein complex isolation and identification of interacting partners and its quantitation;
- Post-translational modification analysis and quantitation;
- Coomassie blue and silver stained protein gel analysis;
- Confirmation of site mutations in protein;
- Post-translational modifications (phosphorylation, sumoylation, ubiquitination, oxidation, etc.);
- Determination of oxidation state of cysteine (disulfide bonds, sulfenic, sulfinic, sulfonic states, etc.);
- Mass determination of intact proteins and other macromolecules;
- Targeted metabolite quantification and profiling by means of LC-MRM-MS analysis. This includes isotope incorporation analysis.
Instruments and technology include:
- SCIEX QTRAP 4000 mass spectrometer integrated with an Agilent 1200 series LC system and a Dionex U3000 nano LC system;
- ThermoFisher LTQ Velos Pro ion trap mass spectrometer system with electron transfer dissociation;
- Protein bioinformatics software (e.g., Bioworks, Metaboanalyst, SEQUEST, Scaffold, Mascot).
